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生物通商城首页 -> 试剂 > 细胞生物学 > 细胞染料/标记示踪 > 细胞骨架

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DyLight? 350?Phalloidin 300units

价格:¥

货号:12848S
品牌:CST

经销:基因有限公司

DyLight?350偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight?350 Phalloidin, 300units

DyLight? 488?Phalloidin 300units

价格:¥

货号:12935S
品牌:CST

经销:基因有限公司

DyLight?488偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight?488 Phalloidin, 300units

DyLight? 554?Phalloidin 300units

价格:¥

货号:13054S
品牌:CST

经销:基因有限公司

DyLight? 554偶联标记鬼笔环肽素,细胞骨架标记荧光染料,300unit
DyLight? 554 Phalloidin,300 units

DyLight? 594 Phalloidin 300units

价格:¥

货号:12877S
品牌:CST

经销:基因有限公司

DyLight?549偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight?594 Phalloidin, 300units

DyLight? 650 Phalloidin 300units

价格:¥

货号:12956S
品牌:CST

经销:基因有限公司

DyLight?650偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit
DyLight?650 Phalloidin, 300units

Alexa Fluor? 488 Phalloidin 300assays

价格:¥

货号:8878S
品牌:CST

经销:基因有限公司

Alexa Fluor? 488偶联标记的鬼笔环肽,细胞骨架标记荧光染料,300unit,Excitation: 495, Emission: 518。 对于6.6 μM (20X)浓度的储存液,将冻干粉溶于1.5 ml纯甲醇中。固定细胞,用新鲜无甲醇的4%甲醛固定15分钟,然后用PBS清洗3次,每次5分钟。一旦固定,根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育,Alexa Fluor? 488鬼笔环肽用PBS 1:20稀释(100μl体积中加入5μl)后加入细胞中。在室温下孵育15分钟后,用PBS清洗一次。盖玻片随着ProLong? Gold Antifade Reagent #9071轻轻滑动,用合适的激发波长检测样品。
Alexa Fluor? 488 Phalloidin, 300assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. ? Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor? 488 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong? Gold Antifade Reagent #9071 and examine specimen using appropriate excitation wavelength.

Alexa Fluor? 647 Phalloidin 300assays

价格:¥

货号:8940S
品牌:CST

经销:基因有限公司

Alexa Fluor? 647标记的鬼笔环肽,细胞骨架标记荧光染料。一种自然出现的有毒的二环肽,存在于剧毒毒伞的子实体菌、鬼笔鹅膏中,对丝状肌动蛋白有很强的亲和力。对于6.6 μM (20X)浓度的储存液,将冻干粉溶于1.5 ml纯甲醇中。固定细胞,用新鲜无甲醇的4%甲醛固定15分钟,然后用PBS清洗3次,每次5分钟。一旦固定,根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育,Alexa Fluor? 647鬼笔环肽用PBS 1:20稀释(100μl体积中加入5μl)后加入细胞中。在室温下孵育15分钟后,用PBS清洗一次。盖玻片上滴加ProLong? Gold 抗淬灭试剂,用合适的激发波长检测样品。
300 assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. ? Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor? 647 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong? Gold antifade reagent and examine specimen using appropriate excitation wavelength.

Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin .

Alexa Fluor?555 Phalloidin 300assays

价格:¥

货号:8953S
品牌:CST

经销:基因有限公司

Alexa Fluor? 555标记的鬼笔环肽,细胞骨架标记荧光染料。一种自然出现的有毒的二环肽,存在于剧毒毒伞的子实体菌、鬼笔鹅膏中,对丝状肌动蛋白有很强的亲和力。对于6.6 μM (20X)浓度的储存液,将冻干粉溶于1.5 ml纯甲醇中。固定细胞,用新鲜无甲醇的4%甲醛固定15分钟,然后用PBS清洗3次,每次5分钟。一旦固定,根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育,Alexa Fluor? 555鬼笔环肽用PBS 1:20稀释(100μl体积中加入5μl)后加入细胞中。在室温下孵育15分钟后,用PBS清洗一次。盖玻片上滴加ProLong? Gold 抗淬灭试剂,用合适的激发波长检测样品。
300 assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. ? Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor? 555 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong? Gold antifade reagent and examine specimen using appropriate excitation wavelength.

Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin .

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